KF 2 ICS Transmitted Light Microscope Operating Manual
Carl Zeiss KF 2 ICS Knowledge of this manual is required for the operation of the instrument. Would you therefore please make yourself familiar with the contents of this manual and pay special attention to hints concerning the safe operation of the instrument. The specifications are subject to change; the manual is not covered by an update service. © Unless expressly authorized, forwarding and duplication of this document, and the utilization and communication of its contents are not permitted.
KF 2 ICS Carl Zeiss CONTENTS Page INTRODUCTION ................................................................................................................. I Title page ............................................................................................................................ I Copyright ........................................................................................................................... II Contents ...............................................................
Carl Zeiss KF 2 ICS Notes on instrument safety The KF 2 ICS microscope, including its original accessories, may only be used for the microscope applications described in this manual. The manufacturer cannot assume any liability for any other applications, possibly also involving individual modules or single parts. This also applies to all service or repair work which is not carried out by authorized service personnel.
KF 2 ICS Carl Zeiss The instrument must be operated by trained personnel only, who must be aware of the possible danger involved with microscopy and the relevant application. The KF 2 ICS microscope is an optical precision instrument which can be impaired in its performance or damaged when handled improperly. All repairs inside the instrument may only be performed by the Carl Zeiss Microscopy Service in Germany (see page 4-6) or your local Carl Zeiss agency.
Carl Zeiss KF 2 ICS Overall view of the KF 2 ICS microscope VI B 40-028 e 10/97
KF 2 ICS Carl Zeiss DESCRIPTION Contents 1 1.1 1.2 1.3 1.4 DESCRIPTION .................................................................................................................1-3 Terms, intended use ........................................................................................................1-3 Instrument description .....................................................................................................1-3 Modules .........................................................
Carl Zeiss 1-2 KF 2 ICS B 40-028 e 10/97
KF 2 ICS Carl Zeiss 1 DESCRIPTION 1.1 Terms, intended use Manufacturer's name: KF 2 ICS microscope The KF 2 ICS microscope is a universal microscope for the visualization of fine structures and forms in biology and medicine. These are typical application fields of this microscope: - Schools, universities - Medicine (for diagnosis) - Quality inspection 1.
Carl Zeiss KF 2 ICS 1.3 Modules Fig.
KF 2 ICS Carl Zeiss KF 2 ICS microscope equipment Cat. No. KF 2 ICS microscope stand with 4-position W 0.8 nosepiece, condenser 0.9 AS with aperture diaphragm and additional lens for low-power observation, built-in illuminator, stabilized power supply, power cable with safety plug or American flat plug, 2 halogen lamps 6V/10W and instrument protection cover. 1.
Carl Zeiss 1.4 (1) KF 2 ICS Technical Data Dimensions Dimensions (width depth height) ......................................................................185 185 400 mm (2) Weight Stand equipment................................................................................................................. approx. 3 kg Binocular tube 45°/20 ICS.................................................................................................... approx. 1 kg KF 2 ICS microscope, complete .......
KF 2 ICS Carl Zeiss START-UP Contents 2 2.1 START-UP.......................................................................................................................2-3 Installation of the instrument ...........................................................................................2-3 List of illustrations Fig. 2-1 Fig. 2-2 Fig. 2-3 Fig. 2-4 Fig. 2-5 Fig. 2-6 Attachment of binocular tube ..........................................................................................
Carl Zeiss 2-2 KF 2 ICS B 40-028 e 10/97
KF 2 ICS Carl Zeiss 2 START-UP 2.1 Installation of the instrument The KF 2 ICS microscope, including accessories, is delivered in standard packaging. We would recommend you to keep the packaging material so that the instrument can be stored for a longer period of time or returned to the manufacturer. (1) Preparations Remove the microscope from the transport case and place it on the worktable.
Carl Zeiss KF 2 ICS (3) Screwing in objectives Remove dust caps (2-2/2) according to the number of objectives and screw objectives (2-2/3) in nosepiece clockwise one by one, starting with the lowest magnification. Nosepiece eyes which are not required should be closed with dust caps. Fig. 2-3 Screwing in objectives (4) Inserting the eyepieces Remove both protection caps (2-4/1) from the binocular tube (2-4/2). Insert the fixed eyepiece PL 10x/18 Br.
KF 2 ICS (5) Carl Zeiss Switching on the line and line/battery modes The illumination system of the KF 2 ICS has been designed for operation using a power unit, but is also available for line/battery operation on request. Subsequent conversion from line operation to line/battery operation is not possible. View the type label (4-2/2) to check whether the built-in lamp supply complies with the present line voltage.
Carl Zeiss b) KF 2 ICS Switch on the line/battery operation Fig. 2-5 Switching on the line/battery operation Line operation Connect KF 2 ICS to line plug via line cable. Set switch (2-6/4) to "Mains ON". Set the required brightness using the "Dimmer Mains" control (2-6/2). Battery operation: Connect plug (2-6/10) of the "Control panel for battery" to socket (2-6/5). Set switch (2-6/4) on the Standard KF 2 ICS in position "Battery ON".
KF 2 ICS Carl Zeiss OPERATION Contents 3 3.1 3.2 3.3 3.4 3.5 3.6 3.7 3.8 3.9 OPERATION ...................................................................................................................3-3 Objective selection...........................................................................................................3-3 Visual overall magnification..............................................................................................3-4 Eyepiece reticles..............................
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KF 2 ICS Carl Zeiss 3 OPERATION 3.1 Choice of objectives The objectives are the centerpiece of the microscope. The following is an example of how objectives can be labeled: CP-"Achromat" 10 /0.25 /where 10 = objective magnification 0.25 = numerical aperture = infinite tube length - = usable for covered and uncovered specimens = only for covered specimens with a cover slip thickness of 0.17 mm or 0.
Carl Zeiss KF 2 ICS 3.2 Visual overall magnification The MM visual overall magnification of the microscope results from the multiplication of the MObj objective magnification with the Meyep. eyepiece magnification: Example: 3.3 = 40 Meyep. = 10 MM 40 10 = 400 MObj = Eyepiece reticles The 40 objective mentioned in the example under 3.2 produces an intermediate image of the object with the magnification 40 : 1.
KF 2 ICS (2) Carl Zeiss Compensating for myopia Two focusing eyepieces PL 10 /18 Br. foc. are required to allow the user to compensate for differences in the visual performance of his two eyes. Use the eyelens of the focusing eyepiece to focus on the line figure of the eyepiece reticle; focus on the edge of the field of view if no eyepiece reticle is used. Focus on the microscope image of a specimen via the focusing drive by looking through the eyepiece with reticle.
Carl Zeiss 3.4 KF 2 ICS Setting the microscope in brightfield Place specimen on the sample stage with the cover slip pointing upwards. The condenser 0.9 AS (3-2/3) is aligned with the specimen stage and firmly attached to it. It contains an aperture diaphragm to control the image contrast. The condenser lever (3-2/1) is attached to the bottom of the condenser via a knurled knob (3-2/4). Swing in the 10x objective.
KF 2 ICS Carl Zeiss Look through the fixed eyepiece of the binocular tube first and focus on the object via the focusing drive (3-3/2). Then set the focus for the other eye by turning the eyelens of the focusing eyepiece (3-3/1). Set the distance of the tubes in such a way that you can see a sharply limited field of view with both eyes. Control the image contrast and the resolving power via the condenser (aperture) stop using the lever (3-2/2). Remove an eyepiece from the tube for checking.
Carl Zeiss 3.5 KF 2 ICS Setting the microscope for phase contrast The condenser lever (3-2/1) can be exchanged for a condenser lever with phase stop Ph 2 (46 52 14) (3-4/3). For this, unscrew the knurled knob (3-2/4), pull out the changer in downward direction, slide in the new changer until stop and fix it via the knurled knob. Set the object in brightfield first.
KF 2 ICS Carl Zeiss Turn the screws (3-4/2) to superimpose the dark Ph ring on the bright ring. The dark phase ring of the objective must fully cover the bright illumination ring. A centering telescope (3-5/1) can also be inserted into the tube instead of a diopter. If a centering telescope is used, its eyelens must be shifted until the illuminating ring is in focus. Insert the eyepiece (3-5/3) in the tube again. Adjust the lamp brightness via control (3-3/3).
Carl Zeiss 3.6 KF 2 ICS Setting the microscope in darkfield The condenser lever (3-2/1) can be exchanged for a condenser lever with darkfield stop D 0.7) (3-7/2). For this, unscrew the knurled knob (3-2/4), pull out the changer in downward direction, slide in the new changer until stop and fix it via the knurled knob. Set the object in brightfield first.
KF 2 ICS 3.7 Carl Zeiss Photomicrography The KF 2 ICS microscope (with phototube) can be changed from observation to photography via the pushrod (3-8/7). Special connectors and the T 2 adapter allow commercially available 35 mm SLR cameras and special microscope cameras (e.g. MC 80 DX) to be attached to the camera port of the KF 2 ICS. For the use of the photomicrography equipment, please observe the relevant separate manuals in addition to the information provided in this manual. Assembly (e.g.
Carl Zeiss 3.8 KF 2 ICS Videomicroscopy Various TV 60 adapters allow 1-chip black-and-white and color cameras to be attached to the KF 2 ICS with phototube. 3.9 Illumination via mirror and condenser 0.9 AS Align (turn and tilt) the flatfield surface of the illumination mirror (3-9/2) until diffuse daylight or the light of a separate lamp illuminates the specimen homogeneously. Adjust the object "brightfield". as described under Swing required objective (3-9/1) in the beam path. Fig.
KF 2 ICS Carl Zeiss CARE, MAINTENANCE AND TROUBLESHOOTING Contents 4 4.1 4.2 CARE, MAINTENANCE AND TROUBLESHOOTING ........................................................4-3 Care and maintenance of the instrument .........................................................................4-3 Troubleshooting and requesting service............................................................................4-4 List of illustrations Fig. 4-1 Fig. 4-2 Fig. 4-3 Changing the fuse for line/battery operation ....
Carl Zeiss 4-2 KF 2 ICS B 40-028 e 10/97
KF 2 ICS Carl Zeiss 4 CARE, MAINTENANCE AND TROUBLESHOOTING 4.1 Care and maintenance of the instrument Maintenance of the KF 2 ICS is limited to the following operations: • Cover the instrument with the dust cover after every use. • Do not install instrument in a damp room. • Remove dust from optical surfaces using a natural hair brush or a rubber blower.
Carl Zeiss 4.2 KF 2 ICS Troubleshooting and requesting service Troubleshooting on the KF 2 ICS is limited to only a few actions: • Checking the line voltage • Changing the fuses for line/battery operation • Change and adjustment of the 6 V / 10 W halogen lamp. (1) Checking the line voltage • Make sure that the required line voltage is available. • Check the line cable and plug for defects.
KF 2 ICS (3) Carl Zeiss Change and adjustment of the 6 V / 10 W halogen lamp. • Place the microscope on its side on the table. • Loosen the knurled screw (4-2/1) and pull out the illuminator (4-2/4). • Remove the halogen lamp (4-3/1) from the metal clamps (4-3/2). • Use the plastic protection bag supplied by the manufacturer to hold the lamp and insert the lamp in the metal clamps (4-3/2). Remove the fingerprints before switching on the lamp.
Carl Zeiss (4) KF 2 ICS Requesting service All repairs of mechanical, optical or electronic components inside the instrument and of the electrical components of the KF 2 ICS may only be performed by Carl Zeiss service staff or specially authorized personnel. To ensure the optimum setting and trouble-free function of your microscope even for a longer period of time, we would recommend you to conclude a service/maintenance contract with Carl Zeiss.
KF 2 ICS Carl Zeiss ANNEX - List of abbreviations .................................................................................................................... A-3 Physical and technical units ......................................................................................................... A-3 EC conformity declaration ...........................................................................................................
Carl Zeiss A-2 KF 2 ICS B 40-028 e 10/97
KF 2 ICS Carl Zeiss List of abbreviations AS aspheric Br. for eyeglass wearers CCD Charge-Coupled Device CSA Canadian Standards Association d diameter DC direct current DIN German industrial standards DX coding system for the storage of electronically legible information, e.g. film speed EEC European Economic Community EN European standards FAA free working distance foc.
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