Specifications

Carl Zeiss KF 2
ICS
3-10 B 40-028 e 10/97
3.6 Setting the microscope in darkfield
The condenser lever (3-2/1) can be exchanged
for a condenser lever with darkfield stop D 0.7)
(3-7/2).
For this, unscrew the knurled knob (3-2/4), pull
out the changer in downward direction, slide in
the new changer until stop and fix it via the
knurled knob.
Set the object in brightfield first. Switch the
lever (3-7/1) in the left position (free passage)
and then move the aperture diaphragm to the
right until it has a suitable opening (also see
section 3.4). For focusing, search for an object
spot featuring minimum structure (if need be, a
spot at the edge of the specimen).
Swing in darkfield stop Ph 2 (3-7/2) by
switching the lever (3-7/1) in the right position.
The aperture diaphragm opens automatically.
To optimize the darkfield setting, the darkfield
stop (3-7/2) can be adjusted in height until the
object appears as bright as possible and the
background as dark as possible.
Set maximum brightness via control (3-3/3).
Optimum darkfield requires extreme cleanliness. Therefore, clean the front lens of the objective
used, the visible condenser surface, the upper cover slip surface and the lower carrier plate
surface with particular care and make them free from grease.
Fig. 3-7 Setting the microscope in darkfield