® ® Luminex FLEXMAP 3D Hardware User Manual For research use only. Not for use in diagnostic procedures.
© Luminex Corporation, 2012. All rights reserved. No part of this publication may be reproduced, transmitted, transcribed, or translated into any language or computer language, in any form or by any means without prior express, written consent of Luminex Corporation. LUMINEX CORPORATION 12212 Technology Boulevard Austin, Texas 78727-6115 U.S.A.
Standard Terms and Conditions for Use of Instrument Product By opening the packaging containing this instrument product ("Product") or by using such Product in any manner, you are consenting and agreeing to be bound by the following terms and conditions. You are also agreeing that the following terms and conditions constitute a legally valid and binding contract that is enforceable against you.
hardware not provided by Luminex. If Product is purchased from a Luminex authorized reseller, any warranty obligations shall be provided in writing directly by such Luminex authorized reseller to Buyer. THIS WARRANTY IS EXCLUSIVE AND LUMINEX MAKES NO OTHER WARRANTY, EXPRESS OR IMPLIED, INCLUDING, WITHOUT LIMITATION, ANY IMPLIED WARRANTY OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, OR NON-INFRINGEMENT.
Luminex's laser based fluorescent analytical test instrumentation. Buyer further acknowledges that the Product has not received approval from the United States Food and Drug Administration or other federal, state or local regulatory agencies and has not been tested by Seller or Luminex for safety or efficacy in food, drug, medical device, cosmetic, commercial or any other use, unless otherwise stated on the Product label or in Seller's technical specifications or material data sheets furnished to Buyer.
consulting fees) that any of the Released Parties may sustain or incur as a result of any claim against such Released Party based upon negligence, breach of warranty, strict liability in tort, contract or any other theory of law or equity arising out of, directly or indirectly, the use of the Product or by reason of Buyer's failure to perform its obligations contained herein.
Table of Contents Chapter 1 Safety ..................................................................................................................1 Description ...................................................................................................................................................1 Warnings and Notes ....................................................................................................................................1 Symbols ......................................
Chapter 3 Maintenance and Cleaning .............................................................................27 General Maintenance Precautions ............................................................................................................27 Daily Maintenance .....................................................................................................................................27 Initializing the Luminex® FLEXMAP 3D® Instrument ...................................................
Appendix D FLEXMAP 3D® Probe Height Adjustment Tool .........................................67 Appendix E Maintenance Schedule .................................................................................71 Short Term Maintenance Schedule ...........................................................................................................71 Long Term Maintenance Schedule ............................................................................................................
Luminex® FLEXMAP 3D® Hardware User Manual x For research use only. Not for use in diagnostic procedures.
Chapter 1: Safety Become familiar with the information in this chapter before using the equipment. Do not perform procedures on your system that are not specifically contained in this manual, unless you are directed to do so by Luminex Technical Support. Description The Luminex® FLEXMAP 3D® system is a life science research multiplex test system intended to measure and sort multiple signals generated in an assay from a biological sample.
Symbols You may encounter these symbols during the use and operation of the Luminex® FLEXMAP 3D® system. They represent warnings, conditions, identifications, instructions, and regulatory agencies. Warning symbols are further explained in the Safety Precautions section.
FIGURE 1. MET Mark In addition, Luminex FLEXMAP 3D complies with European Union (EU) safety requirements and therefore may be marketed in the Europe Single Market. The following European Union compliance label appears on the back of the FLEXMAP 3D instrument. FIGURE 2. European Union Compliance Label Safety Precautions Read the following safety information before using the Luminex® FLEXMAP 3D® instrument.
WARNING: Do not use this device in close proximity to sources of strong electromagnetic radiation, for example, unshielded intentional RF sources, as these may interfere with proper operation WARNING: Always handle the FLEXMAP 3D® system according to Luminex instructions to avoid any possible interference from its electromagnetic fields. Lasers The Luminex® FLEXMAP 3D® instrument is classified under FDA 21 CFR 1040.10 and 1040.
of procedures other than those specified in this manual can result in hazardous radiation exposure. The bar code reader laser presents a potential hazard to eyesight. DANGER: Do not stare into the barcode reader beam or shine it into other people’s eyes. Fluidics This instrument contains fluids. In the event of a fluid leak, turn off all power to the system and disconnect all power cords. The on/off switch is not a method of disconnection; the power cord must be removed from the outlet.
Mechanical Components WARNING: The Luminex® FLEXMAP 3D® instrument has parts that move during operation. Risk of personal injury is present. The moving parts present puncture, pinching, and hand-crushing hazards. Keep your hands and fingers away from the XY slot, syringe pumps, and sample probe during operation. Observe all warnings and cautions. Keep the access doors closed during normal operations.
FIGURE 5. Fuse Caution Label The following voltage label appears on the back of the FLEXMAP 3D® instrument. It displays the FLEXMAP 3D® serial number, model number, power requirements, and manufacturer’s information. FIGURE 6. Serial Number and Voltage Label Heat The heater plate, used to warm the heater block of the XY platform, can be heated between 35°C and 60°C. WARNING: The heater plate of the Luminex® FLEXMAP 3D® XY platform may be hot and can cause personal injury if touched.
WARNING: Wear appropriate personal protective equipment when handling parts that come into contact with potentially biohazardous samples. To decontaminate the FLEXMAP 3D® instrument: 1. Remove all specimens and all FLEXMAP 3D® reagents. 2. Use the software to run a sanitize command with the diluted (10% to 20%) household bleach solution. Leave deionized water and household bleach solution diluted to 10% to 20% in water in the system. 3. Use the software to run two wash commands with deionized water.
Disposal of Instrument NOTE: Within the European Union and other jurisdictions, in accordance with Directive 2002/96/EC of the European Parliament and of the Council regarding Waste Electrical and Electronic Equipment (WEEE), you must properly dispose of electrical and electronic equipment when it reaches its end of life. If you are disposing of a Luminex® FLEXMAP 3D® instrument, decontaminate the system. See the section titled “Decontamination Procedure”.
Luminex® FLEXMAP 3D® Hardware User Manual 10 For research use only. Not for use in diagnostic procedures.
Chapter 2: Technical Overview This chapter reviews the technical aspects of the Luminex® FLEXMAP 3D® system: operation, system components, subsystems, recommended additional equipment, and specifications. How the FLEXMAP 3D System Operates The Luminex® FLEXMAP 3D® system, in combination with xMAP® (Multi-Analyte Profiling) technology, will simultaneously measure up to 500 analytes from a single sample.
Subsystems The Luminex® FLEXMAP 3D® instrument includes four subsystems: electronic, fluidic, mechanical, and optical. The following topics describe the user-accessible components of each subsystem. Electronics The electronics system provides the power for operation and control of the FLEXMAP 3D® system and communication between its parts. Power Input Module The power input module contains the input power plug and fuses. This is the protective earthing point for the Luminex® FLEXMAP 3D® system.
FIGURE 8. Back of the FLEXMAP 3D® Instrument 1 Power Input Module 2 Communications Port P1 (USB type B) Fluidics The fluidics system handles the flow of liquid through the Luminex® FLEXMAP 3D® instrument. Access Doors The Luminex® FLEXMAP 3D® system has two front access doors. The left door supplies access to a door ventilation filter and the sheath filter. The right door supplies access to a door ventilation filter, the sample probe, and the syringe pumps.
FIGURE 9. Front of the FLEXMAP 3D® Instrument 1 Left door, with access to sheath filter and door ventilation filter 2 Right door, with access to syringe pumps, door ventilation filter, and sample probe Sample Probe A stainless steel sample probe acquires the sample. See the "Sample Probe" image below. WARNING: Avoid contact with moving parts. Cheminert® Fitting This fitting attaches the sample probe to the sample tubing. Disconnect this fitting when you remove the sample probe.
FIGURE 10. Sample Probe 1 Cheminert fitting 2 Attachment point on Cheminert fitting for sample tubing 3 Sample probe, in up position Syringe Pump The syringe delivers a sample from the microtiter plate to the cuvette. See the "Syringe Pump" image below. WARNING: Avoid contact with moving parts. For research use only. Not for use in diagnostic procedures.
FIGURE 11. Syringe Pump 1 Syringe arm (within dotted lines) 2 Glass syringe barrel 3 Syringe plunger Sheath Filter The sheath filter removes particles greater than ten microns in diameter from the sheath fluid. See the "Fluidics Bay" image below. Fluidics Bay The waste and sheath connectors, located on the front of the fluidics bay, connect to the sheath fluid and waste fluid containers using clear tubing. The sheath fluid connector is blue and the waste connector is orange.
FIGURE 12. Fluidics Bay 1 Sheath filter 2 Blue tubing attached to sheath fluid container 3 Orange tubing attached to waste fluid container Waste Fluid Container The waste fluid container receives waste from the system. To maintain a stable flow rate, do not move the waste line or container during system operation and make certain the waste fluid container is properly vented. Place the container at least three feet below the surface on which the instrument rests.
of this manual. The "Fluidics Bay" image above and "Front Door Ventilation Filters" image below show the location of these filters. FIGURE 13. FIGURE 14. Front Door Ventilation Filters View A Right front door, viewed from inside View B Left front door, viewed from inside 1 Filter 2 Holding clamp XY and HEPA Filters View A Front of XY, with XY cover removed View B Front of fluidics bay, behind left front door 1 XY filter Luminex® FLEXMAP 3D® Hardware User Manual 18 For research use only.
TABLE . Continued 2 Holding clamp 3 Cover over HEPA air filter Optics The optical system consists of the optical assembly and the excitation lasers. The optical components do not require manual adjustment by the user. System Components The following topics describe details of the three components of the Luminex® FLEXMAP 3D® system: software, reagents, and hardware.
CAUTION: Adhere to standard laboratory safety practices when handling hazardous, toxic, or flammable reagents and chemicals. Contact Luminex Technical Support when in doubt about compatibility of cleaning and decontamination agents or materials. Required Laboratory Reagents • 10 - 20% household bleach • 70% isopropanol or 70% ethanol • 0.1N NaOH • Sporicidin® Disinfectant • Mild detergent • Deionized water WARNING: Isopropanol and ethanol are flammable liquids.
CAUTION: Luminex calibrators and verifiers contain sodium azide. Sodium azide is toxic and may also react with lead and copper plumbing to form highly explosive metal azides. On disposal, flush drains with a generous amount of cold water to prevent azide build-up. Consult the manual guideline Safety Management No. CDC-22, Decontamination of Laboratory Sink Drains to remove Azide Salts (Centers for Disease Control, Atlanta, Georgia, April 30, 1976).
Printer Use a printer compatible with Microsoft® Windows XP SP3. Barcode Labels Use Code 128 barcode label type when scanning barcode labels into the system. Vortex Use VWR product number 58816-12, with a speed range of 0 to 3200 rpm, or equivalent. Bath Sonicator Use Cole-Parmer® product number 08849-00, with an operating frequency of 55 kHz, or equivalent.
TABLE . Continued Temperature control: Maintains samples using the heater block at a constant temperature from 35°C to 60°C (95°F to 131°F), +/- 1°C of setpoint. Altitude: Operation up to 2400 m (7874 ft.
Electronics USB 2.0-compatible communications link for fast data transfer Input voltage range: 100-120 V~, 6.0 A, 50/60 Hz or, 200-240 V~, 3.0 A, 50-60 Hz Optics Reporter channel detection: A/D resolution 16 bits Reporter channel dynamic range: ≥ 4.5 decades of detection (verified with beads dyed with high levels of organic dyes) Reporter laser: 532 nm, nominal output 15.
TABLE . Continued Detects and distinguishes surface reporter fluorescence emissions at 575 nm on the surface of 1 to 500 unique xMAP microspheres in a single sample Soluble background fluorescence emission at 575 nm automatically subtracted from fluorescence intensity values.
Luminex® FLEXMAP 3D® Hardware User Manual 26 For research use only. Not for use in diagnostic procedures.
Chapter 3: Maintenance and Cleaning To ensure accurate test results, properly clean and maintain the Luminex® FLEXMAP 3D® system. Read and follow all instructions in this section. Perform adequate maintenance and cleaning to avoid inaccurate results and potential hazards. To facilitate your maintenance process, print out and use the maintenance logs found in the Maintenance Log section.
the performance of these activities, see the appropriate Luminex software manual or Luminex online help. Initializing the Luminex® FLEXMAP 3D® Instrument NOTE: Use the software to perform system initialization. The software provides three options for system initialization: • Warmup, fluidics • Warmup, fluidics, verification • Warmup, fluidics, verification, and calibration Select the option you want.
WARNING: Do not move the waste line vertically while the FLEXMAP 3D® instrument is running. Contact Luminex Technical Support before relocating the waste fluid container or rerouting the waste line. You can move the line temporarily for cleaning and maintenance. To empty the waste fluid container: 1. Disconnect the waste fluid container from the FLEXMAP 3D® instrument. 2.
Removing Clogs NOTE: If you frequently use the Luminex® FLEXMAP 3D® system to test concentrated serum or other debris ridden samples, Luminex recommends that you use the software to perform a clog removal routine weekly. Luminex recommends using sodium hydroxide (NaOH) to remove clogs. To remove clogs: 1. Add 0.1N sodium hydroxide solution to reservoir RB1 on the off-plate reagent block. 2. Use the software to perform a clog removal routine. WARNING: Sodium hydroxide is extremely caustic.
4. Replace the sample probe and tightly screw in the Cheminert fitting. 5. Use the software to perform an automatic probe height adjustment. NOTE: Perform an automatic probe height adjustment any time the probe is removed. NOTE: For information on calibrating the probe correctly, refer to the xPONENT Software User Manual. FIGURE 15. The Sample Probe 1 Cheminert Fitting - Unscrew as shown in Step 2. 2 Probe Holder 3 Sample Probe - Push up and out of probe holder in Step 2.
Monthly Maintenance Clean the exterior surfaces monthly. To clean exterior surfaces: 1. Turn off the Luminex® FLEXMAP 3D® instrument and unplug the power cord. 2. Wipe all exterior surfaces with mild detergent, followed by a household bleach solution diluted to 10% to 20%, followed by deionized water. 3. Open both doors of the instrument. 4. Clean all accessible surfaces with detergent, followed by the household bleach solution (10% to 20%), followed by deionized water.
9. Screw the syringe back into its housing. 10. Return the syringe arm to its original position. The bottom of the plunger fits into the indentation in the syringe arm. 11. Hand-tighten the set screw on the syringe arm. 12. Plug in the power cord and turn on the FLEXMAP 3D instrument. 13. Use the software to run the prime command twice, watching for any leaks in the syringe area. 14. Close the right front door. FIGURE 16.
Replacing the HEPA Air Filter The HEPA air filter is the round filter behind the panel on the left side of the front of the fluidics bay. Tubing from the interior of the fluidics bay is attached to the stem in the center back of the filter. To replace the HEPA air filter: 1. Turn off the Luminex® FLEXMAP 3D® instrument and unplug the power cord. 2. Open the left door and locate the HEPA air filter panel on the front of the fluidics bay as shown in the HEPA Air Filter image below. 3.
Cleaning the Ventilation Filters Each filter has at least one imprinted arrow on its metal frame. This arrow indicates the direction of the air flow. Filters should be installed so that the arrows are at the correct locations and point in the correct direction. To clean the Luminex® FLEXMAP 3D® ventilation filters: 1. Turn off the FLEXMAP 3D® instrument and unplug the power cord. 2. Remove the XY cover by gently grasping its ventilation holes and pulling it straight out. 3.
8. Reinstall the XY cover and close the access doors. 9. Plug in the power cord and turn on the FLEXMAP 3D® instrument. FIGURE 18. Ventilation Filter on XY Platform View A Front of the FLEXMAP 3D® instrument with XY cover in place View B Front of the FLEXMAP 3D® instrument with XY cover removed 1 XY Cover (inside dotted lines) 3 Filter 2 Location of ventilation holes on top of XY cover (visible only when doors are open) 4 Filter Clamp 5 Incised Arrow FIGURE 19.
View A Right front door, viewed from inside 2 Filter Clamp View B Left front door, viewed from inside 3 Incised Arrow 1 Filter Annual Maintenance Annual maintenance requires replacing the sheath filter. To replace the Luminex® FLEXMAP 3D® instrument sheath filter: 1. Turn off the FLEXMAP 3D® instrument and unplug the power cord. 2. Open the left door on the FLEXMAP 3D® instrument and locate the sheath filter on the front of the fluidics bay. 3.
6. Plug in the power cord and turn on the FLEXMAP 3D® instrument. 7. Use the software to run the prime command twice. FIGURE 20. Front of Fluidics Bay (Inside Left Door) 1 Top quick-disconnect point 2 Sheath Filter 3 Bottom quick-disconnect point Storing the Luminex® FLEXMAP 3D® Instrument If you need to put the Luminex® FLEXMAP 3D® instrument in long-term storage or prepare it for use after removing it from long-term storage, use the following procedures.
2. Remove the sample probe from the instrument and flush it with deionized water from the narrow end out through the larger end. 3. Replace the sample probe in the sample arm and wrap the end of the probe with Parafilm®. Preparing the Luminex® FLEXMAP 3D® Instrument for Use After Storage Follow this procedure before you begin using the Luminex® FLEXMAP 3D® instrument after its removal from long-term storage. To prepare the FLEXMAP 3D® instrument for use after storage: 1.
4. Check both of the fuses in the cartridge for damage. A fuse can display physical evidence of damage, for example, broken wire or blackened glass; if it displays no physical evidence, test it for continuity with a voltmeter. 5. Replace damaged fuses with the type specified on the sticker to the right of the power input module. 6. Replace the red cartridge. 7. Shut the module door. 8. Plug in the power cord and turn on the FLEXMAP 3D® instrument.
maintenance (one task monthly), semi-annual maintenance (two tasks twice yearly), and annual maintenance (two tasks yearly). For each item listed at the left, enter your initials under each date on which you perform the task. Use the Maintenance Logs (located in Appendix E) to record maintenance information. In the Short Term Maintenance Schedule, the first table includes a sufficient number of columns for daily maintenance recordings for one week (7 days).
Luminex® FLEXMAP 3D® Hardware User Manual 42 For research use only. Not for use in diagnostic procedures.
Chapter 4: Troubleshooting Troubleshooting procedures help users identify and remedy problems with the instrument. Overview To troubleshoot a problem, locate the symptom in one of the tables in this chapter, determine the cause from the listed possibilities, and remedy it with the provided solution.
Power Supply Problems Power supply problems often involve a blown fuse, faulty electronic component, or disconnected cable. CAUTION: Whenever you deal with a potential electrical problem, be careful to avoid electrical shocks. TABLE 1. Power Supply Problems Symptom ® ® The Luminex FLEXMAP 3D instrument will not turn on. Fuses continue to open (blow). Possible cause Solution The power cord is disconnected. Plug in the power cord. No voltage is coming from the electrical outlet.
Clogs Often, a clog somewhere in the Luminex® FLEXMAP 3D® instrument is the cause of a fluid leak, a pressurization or sample probe problem, or a problem with calibration, verification, or data acquisition. To determine whether there is a clog, check the bead count during calibration. During calibration you should see 300 bead events per second or more. If there are fewer than that, there is probably a clog in the instrument.
Fluid Leaks Fluid leaks can result in poor pressurization and failed sample acquisition. TABLE 4. Fluid Leaks Symptom Possible cause Solution Fluid is pooled around the Luminex® FLEXMAP 3D® instrument. Fittings or fluid lines are damaged. Turn off and disconnect the instrument to avoid electrical shock and contact Technical Support. Fluid drips from the sample probe. The sample probe is clogged. Fluid leaks from the front of the instrument. See the section titled “Clogs”.
TABLE 5. Sample Probe Problems Continued Symptom Possible cause Solution The microtiter plate is warped. Inspect the microtiter plate. Replace it if it is warped. The sample probe is bent. Remove the sample probe from the instrument. Roll it on a flat surface. If a sample probe has been bent and rolled straight more than once, discard it and replace it with a new sample probe. Perform an automatic sample probe height adjustment using the software. Calibration Problems TABLE 6.
TABLE 6. Calibration Problems Continued Symptom No events are collected during calibration. Possible cause Solution There is a problem internal to the instrument. Review the log of calibration reports. Check for dramatic changes in temperature, sheath pressure, or voltage. If any of these are present, contact Technical Support. There is a problem with fluid levels. Check the sheath and waste fluid levels. Verify that tubing for both bottles is tightly connected to the instrument.
TABLE 7. Verification Problems Continued Symptom Possible cause Solution The waste line was moved during instrument operation, resulting in an unstable flow rate. Stabilize the waste line during instrument operation. See the section titled “Maintaining Fluids”. There is a problem internal to the instrument. Review the log of calibration reports. Check for dramatic changes in temperature, sheath pressure, or voltage. If any of these are present, contact Technical Support.
TABLE 8. Verification Problems Continued Symptom Possible cause Solution The microspheres are photobleached. Replace the microspheres with unphotobleached microspheres. There are an insufficient number of beads in the sample. Ensure that there are 2000 to 5000 beads per bead set per well. The sample is too concentrated. Dilute concentrated biological fluids, such as serum or plasma, at least 1:5.
TABLE 9. Symptom Bead Detail Irregularities Description Possible Problem Solution xMAP and calibrator microspheres form a group in the wrong location. Calibrator microspheres group The calibrator microspheres together at the top of the are photobleached. region. Replace the calibrator microspheres with unphotobleached calibrator microspheres. To avoid photobleaching, protect microspheres from light. xMAP microspheres group together at the lower right of the region.
TABLE 9. Symptom Bead Detail Irregularities Continued Description Possible Problem Solution xMAP microspheres do not There is air in the instrument. form a cohesive group and are somewhat scattered. Verify the sample probe height. Use the software to run a prime command three times, an alcohol flush command twice, then a wash command three times with deionized water. xMAP microspheres are widely scattered. Make sure there is sheath fluid in the sheath container.
Appendix A: Shipping Shipping If a serious problem arises with the Luminex® FLEXMAP 3D® system, it may be necessary to return it to Luminex Corporation for repairs. If Luminex Technical Support directs you to return the FLEXMAP 3D® system, the Technical Support representative will provide you with all necessary information as well as give you a Return Material Authorization (RMA) number.
6. Connect the waste fluid tubing (orange connector) to the bottom quick disconnect point (white connection) of the sheath filter tubing. 7. Use the software to run a warmup command. This starts the compressor, which drains the sheath fluid from the reservoir into the waste fluid container. NOTE: To refill the reservoir, reconnect each connector to its color-matched connection and use the software to run a prime command. FIGURE 23.
Yes No Printed Name _____________________________________________________ Signature ________________________________________________________ Company/Institution ________________________________________________ Date________________ Instrument Serial No.___________________________ For research use only. Not for use in diagnostic procedures.
Luminex® FLEXMAP 3D® Hardware User Manual 56 For research use only. Not for use in diagnostic procedures.
Appendix B: Installation Installation of the Luminex® FLEXMAP 3D® The following installation instructions for Luminex® FLEXMAP 3D® are to be used for site prep, assessing facilities requirement and in the event cabling or tubing must be reconnected. NOTE: If you do not intend to keep the packing material for future use, return the packaging of the product, spare parts, and options to the manufacturer with the Field Service Engineer.
2. Connect the FLEXMAP 3D® instrument and all necessary peripheral devices to the PC. FIGURE 24. Back of PC 1. Monitor Plug 5. Monitor Plug (Touchscreen monitors only) 2. FLEXMAP 3D Communications Plug (USB type A ) 6. Stereo Speaker Power (USB connection) 3. Mouse Plug 7. Stereo Speaker Audio Connection 4. Barcode Scanner Plug 8. Power Inlet FIGURE 25. Back of FlexMAP 3D Instrument Luminex® FLEXMAP 3D® Hardware User Manual 58 For research use only.
1. Power Inlet 2. PC Communications plug (USB type B) 3. Connect the USB communications cable between the FLEXMAP 3D® instrument (P1) and the PC, and connect the power cables for the PC, monitor, and FLEXMAP 3D® instrument to outlets but do not turn on any of the devices. 4. Place the sheath container below the instrument with the container opening facing up. 5. Remove the existing container cap and install the cap from the blue tubing accessory in its place. 6.
18. Calibrate the probe height using the instructions in the software user manual. NOTE: For information on calibrating the probe height when using softbottom 384-well plates, refer to the FLEXMAP 3D 384-Well Plate Probe Height Adjustment Tool section. 19. Calibrate the instrument using the instructions in the software user manual. Luminex® FLEXMAP 3D® Hardware User Manual 60 For research use only. Not for use in diagnostic procedures.
For research use only. Not for use in diagnostic procedures.
Luminex® FLEXMAP 3D® Hardware User Manual 62 For research use only. Not for use in diagnostic procedures.
For research use only. Not for use in diagnostic procedures.
Luminex® FLEXMAP 3D® Hardware User Manual 64 For research use only. Not for use in diagnostic procedures.
Appendix C: Part Numbers Hardware Product Description Customer Number Luminex® FLEXMAP 3D® with xPONENT FLEXMAP-3D-RUO Heater Block for 96-well microtiter plates CN-0224-01 Heater Block for 384-well microtiter plates CN-0223-01 Bar Code Scanner CN-PC03-01 Air Filter: Access Door CN-0222-01 Air Filter: XY Platform CN-0229-01 Air Filter: HEPA CN-0228-01 Syringe Cylinder with seal CN-0013-01 Syringe Seal (Quantity 4) CN-0014-01 Sheath Filter with quick disconnect CN-0010-01 Sample Probe
Software Product Description Customer Number ® Luminex xPONENT Software for FLEXMAP 3D CN-SW16-01 Reagents Product Description Customer Number xMAP Sheath Fluid, LX100 40-50000 ® FLEXMAP 3D Calibration Kit F3D-CAL-K25 Microspheres, F3DCAL1 - Classification Calibrator F3DCAL1-05 Microspheres, F3DeCAL1 - Classification Calibrator F3DeCAL1-05 Microspheres, F3DCAL2 - Reporter Calibrator F3DCAL2-05 Microspheres, F3DCAL3 - EDR Calibrator F3DCAL31-05 FLEXCAP 3D Performance Verification Kit F3D-
Appendix D: FLEXMAP 3D® Probe Height Adjustment Tool NOTE: The Luminex® FLEXMAP 3D® Probe Height Adjustment Tool is to be used for 384 well mylar bottom and 384 well filter bottom plates only. To calibrate the probe correctly, perform the following steps: 1. Using data sheet for the plate you intend to calibrate the probe to, subtract the "well depth" from the "plate height" as shown in the example below: Plate Height: 14.2mm - Well Depth: 11.43mm = 2.77mm (2.8mm) Example 1: 2.
2. Click Eject from the System Status bar to eject the XY platform. 3. Place the probe height adjustment tool on the XY platform. 4. From the Home page click Probe and Heater. 5. On the Probe and Heater page, select the plate type (384 well) and enter a plate name. 6. Select the appropriate well to calibrate probe height (C7 for the example shown above). 7. Click Auto Adjust Height. 8. Click Save to save the plate settings. Example 2: "C" Plate Height 14.4mm - "F" Well Depth 11.5mm = 2.9mm 2.
For research use only. Not for use in diagnostic procedures.
Luminex® FLEXMAP 3D® Hardware User Manual 70 For research use only. Not for use in diagnostic procedures.
Appendix E: Maintenance Schedule Short Term Maintenance Schedule 71
Luminex® FLEXMAP 3D® Hardware User Manual 72 For research use only. Not for use in diagnostic procedures.
Appendix E: Maintenance Schedule Long Term Maintenance Schedule 73
Luminex® FLEXMAP 3D® Hardware User Manual 74 For research use only. Not for use in diagnostic procedures.
Appendix E: Maintenance Schedule 75
Luminex® FLEXMAP 3D® Hardware User Manual 76 For research use only. Not for use in diagnostic procedures.